摘要： 为了研制促进月季生长的木霉生防菌肥，采用本实验室分离鉴定的哈茨木霉T6施于月季盆栽土壤中，分析木霉菌对”十姐妹”月季硬枝扦插发芽、幼苗生长、防御酶活性和内源激素水平的影响。结果表明：施用哈茨木霉T6的月季硬枝出芽率和幼苗枝条长度分别是对照的1.4和1.82倍；并且枝条和叶的干重、枝条和叶的含水率均显著增加。此外显著提高了叶中3种防御酶SOD、POD、CAT的活性及6种内源激素IAA、GA3、ZT、ABA、SA和JA的水平。说明木霉T6具有促进月季生长和提高其抗性的作用，研究结果为研制促进月季生长的生物菌肥提供理论依据。
关键词: 哈茨木霉T6, 月季, 生长促进, 防御酶活性, 激素水平
Abstract: In order to develop the biocontrol fertilizer of Trichoderma for promoting growth of Rosa chinensis ‘Shi-Jie-Mei’, with Trichoderma harzianum T6 isolated and identified in our laboratory, the effects of Trichoderma T6 on the sprout of hard branches, seedling growth, the activity of defendant enzyme and the levels of endogenous hormone of rose were analyzed. T.harzianum T6 significantly increased the germination rate of hard branches and the length of the branches of rose seedlings, 1.4 and 1.82 times that of the control group. The dry weight and the water content in branches and leaves of rose were significantly increased. The activity of SOD, POD, CAT and the levels of hormone, including IAA, GA3, ZT, ABA, SA and JA in the leaves, were all significantly increased. The results displayed that T.harzianum T6 had the action of growing promotion on rose and enhancing its resistance, which would provide the theoretical basis for the development of biological fertilizer for rose growth promotion.
Key words: Trichoderma harzianum T6, Rosa chinensis ‘Shi-Jie-Mei’, growth promotion, defense enzyme activity, endogenous hormone level
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