摘要： 二氢杨梅素和杨梅素是拐枣种子中的重要成分。通过超声辅助法从拐枣种子中提取二氢杨梅素，采用单因素法考察乙醇体积分数、超声辐照功率、提取温度、液料比和超声辐照时间影响参数的基础上，并选用Box-Behnken响应面设计法分析建立了超声辐照功率、超声辐照时间和液料比的二次多项式模型，优化提取工艺。结果得到超声辅助法提取拐枣种子中二氢杨梅素的最佳工艺参数为：乙醇体积分数为60%、超声辐照功率140 W、超声辐照时间30 min、液料比20.5 mL·g-1，提取温度40℃。在此最佳条件下，二氢杨梅素得率为2.14±0.09 mg·g-1。本提取方法简单快速，效率高，有利于拐枣资源的综合加工利用。
关键词: 拐枣, 种子, 超声辅助提取, 二氢杨梅素, 响应面
Abstract: Dihydromyricetin is an important component in the seeds of Hovenia dulcis Thunb. The extraction of dihydromyricetin from seeds of H.dulcis by ultrasound-assisted method was carried out on the basis of single factor investigation of ethanol volume fraction, ultrasound irradiation power, extraction temperature, liquid-solid ratio and ultrasound irradiation time. The Box-Behnken response surface design method was used to establish a quadratic polynomial model of ultrasound irradiation power, ultrasound irradiation time and liquid-solid ratio, and optimized extraction process. The optimum conditions for the extraction were as follows:ethanol volume fraction of 60%, ultrasonic irradiation power of 140 W, ultrasonic irradiation time of 30 min, liquid-solid ratio of 20.5 mL·g-1, and extraction temperature of 40℃. Under these optimal conditions, the yield of dihydromyricetin was 2.14±0.09 mg·g-1. The extraction method is simple, rapid, and high in efficiency, which is beneficial to the comprehensive processing and utilization of the H.dulcis resources.
Key words: Hovenia dulcis Thunb., seed, ultrasound-assisted extraction, dihydromyricetin, response surface methodology
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